The 2009 AfterEllen.com Hot 100

41. Natalie Portman

(No. 27 in 2008)

42. Missy Higgins*

(No. 48 in 2008)

43. Piper Perabo

(No. 32 in 2008)

44. Renee O’Connor

(No. 21 in 2008)

45. Gabrielle Christian

(No. 25 in 2008)

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